IN VITRO MICROCLONAL PROPAGATION OF OLIVE (OLEA EUROPAEA L.) PLANT RESISTANT TO STRESS FATORS
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This article provides brief information about in vitro cultivation and microclonal propagation of olive varieties introduced to our country from abroad. According to the study results it was found out that in laboratory apical sterilization of plants, 0.1% sodium hypochlorite was used for sterilization for 25 minutes and in the nutrient media a pH value was 5.55, the number of buds died under the influence of 0.1% sodium hypochlorite was 7 pieces, the number of buds damaged by pathogenic microorganisms was 12 pieces, and the number of surviving buds was 61 pieces, i.e. 76.25%.
During in vitro microclonal propagation, when phytoregulators MMT 1.5 mg/l + IBA 0.01 mg/l were added to MS nutrient media and the pH indicator in nutrient media was 5.60, shoot branching was observed to be 3 pieces in 7-30 days, and the length of branched plants was 3.2 cm.
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References
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