Regeneration of Sorghum through Tissue Culture Techniques
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Abstract
This is the first study in East and Central Africa for optimization tissue culture protocol of sorghum. The effect of hormones on callus induction of sorghum and finally to assess the regeneration response of sorghum through tissue culture techniques. Callus induction and regeneration of four sorghum varieties (Tabat, Elwafer, Yrwasha and Dwarf white - Mylo) was evaluated using mature embryos as source of explants and different concentration of the hormone 2,4-D (0, 1, 2, 4, 6, mg/L) supplemented with 0.2 mg/L Kinetin. The highest callus induction frequency was observed in 2 mg/L for Elwafer and D.W. Milo while 6 mg/L of 2,4-D level gave higher callus induction frequency for Tabat and Yrwasha. The lowest callus induction frequency was observed in 0 and 1 mg/L of 2,4-D level for all the varieties. The highest embryogenic callus induction frequency was observed in Tabat at 4 mg/L and 6 mg/L of 2,4-D while the lowest embryogenic callus induction frequency was observed in 0 and 1mg/L of 2,4-D. Regeneration efficiency was observed higher in the variety Tabat at 4 mg/L of 2,4-D level.
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References
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